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Lund Protein Production Platform (LP3)

Lund University
Lund University

Description

Lund Protein Production Platform, LP3, is a cross-faculty center of Lund University for protein production, purification, crystallisation and structure determination. For more details about LP3, please see its homepage: www.lu.se/lp3

Tags
Bio-based materials and polymers, Chemistry and Catalysis, Medtech - Biomedical engineering, Pharma and Biotechnology, Surfaces and Interfaces

Offer / Action

LP3 offers services for the entire process chain of production, purification, characterisation, protein crystallisation and protein structure determination and structure refinement or each individual step in the chain. LP3 can help with:

  • Plasmids for protein production
  • Recombinant protein production in bacterial (E. coli) or eukaryotic (insect) cells.
  • Protein labeling (seleno-methionine incorporation, labeling with stable isotopes (2H, 13C, 15N), biotinylation, phosphorylation)
  • Protein purification
  • High-throughput  & nanovolume protein crystallisation
  • Biophysical protein characterization by Size Exclusion Chromatography (SEC), Dynamic Light Scattering (DLS) and Differential Scanning Fluorimetry (DSF)
  • Automated crystallisation plate storage and imaging
  • Protein structure determination and refinement
  • Application for beamtime. LP3 is part of a Block Allocation Group (BAG) for BioMAX.
  • MX data collection at synchrotron beamline (BioMAX MAX IV)
  • Process x-ray data and determine and refine the structures
  • Cryo-EM screening, LP3 is part of a Block Allocation Group (BAG) for SciLifeLab Cryo-EM in Stockholm and Umeå

For details of current services and updates, please see the homepage: www.lu.se/lp3

Contact

Wolfgang Knecht
Manager LP3

lp3@biol.lu.se