Lund Protein Production Platform, LP3, is a cross-faculty center of Lund University for protein production, purification, crystallisation and structure determination. For more details about LP3, please see its homepage: www.lu.se/lp3
LP3 offers services for the entire process chain of production, purification, characterisation, protein crystallisation and protein structure determination and structure refinement or each individual step in the chain. LP3 can help with:
Plasmids for protein production
Recombinant protein production in bacterial (E. coli) or eukaryotic (insect) cells.
Protein labeling (seleno-methionine incorporation, labeling with stable isotopes (2H, 13C, 15N), biotinylation, phosphorylation)
High-throughput & nanovolume protein crystallisation
Biophysical protein characterization by Size Exclusion Chromatography (SEC), Dynamic Light Scattering (DLS) and Differential Scanning Fluorimetry (DSF)
Automated crystallisation plate storage and imaging
Protein structure determination and refinement
Application for beamtime. LP3 is part of a Block Allocation Group (BAG) for BioMAX.
MX data collection at synchrotron beamline (BioMAX MAX IV)
Process x-ray data and determine and refine the structures
Cryo-EM screening, LP3 is part of a Block Allocation Group (BAG) for SciLifeLab Cryo-EM in Stockholm and Umeå
For details of current services and updates, please see the homepage: www.lu.se/lp3