Protein Production Sweden (PPS) is a national infrastructure financed by the Swedish Research Council. The universities that are part of the infrastructure are the University of Gothenburg (host), Lund University, KTH, Umeå University and Karolinska Institute. PPS is focused on the production and purification of protein reagents for Swedish researchers. We work with a large variety of expression systems to allow the production of many different types of proteins and can help out with all the different steps involved in the production. Additionally we offer Gateway environments to facilitate reagent production and experimentation at synchrotron and neutron infrastructures. For more information please see www.gu.se/pps
PPS focuses on the production and purification of (mainly) recombinant protein reagents for all Swedish researchers. PPS uses 6 different expression systems which allows the production of many types of proteins. Researchers can access the PPS infrastructure via a common entry-point and obtain individualized support based on their research needs throughout the whole process of protein production and purification. This can include all steps from project counselling and initial design to final quality control, or any single/multiple step(s) of the process as depicted in the Figure above.
PPS also features 2 expertise and development heavy Gateway modules in to support users of large-scale infrastructures which allow preparation of reagents and/or provide expertise for the subsequent performance of experiments: (Per)deuteration for neutron scattering, but also NMR applications and the module Molecular chaperones featuring the concept of Affinity Probe-Enabled Structural Analysis to enable structural analysis by X-ray crystallography, but also Cryo-EM.